The former New York City mayor promptly endorsed former vice-president Joe Biden as the Democrat best placed to take on President Donald Trump in November.
"While I will not be the nominee,I will not walk away from the most important political fight of my life,"Bloomberg said in a statement on Wednesday morning (Thursday AEDT).
"I’ve always believed that defeating Donald Trump starts with uniting behind the candidate with the best shot to do it.
"After yesterday’s vote,it is clear that candidate is my friend and a great American,Joe Biden."
Bloomberg soared in the polls in February thanks to his huge advertising spend,and with moderate Democrats desperately seeking a viable alternative to Senator Bernie Sanders.
But Bloomberg put in a universally panned performance in his first debate and struggled to answer questions about the controversial stop-and-frisk policing practice he championed as mayor.